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KMID : 0545120080180020365
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 2 p.365 ~ p.368
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Regulation of Cav3.2 Ca2+ Channel Activity by Protein Tyrosine Phosphorylation
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Huh Sung-Un
Kang Ho-Won
Park Jin-Yong
Lee Jung-Ha
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Abstract
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Calcium entry through Cav3.2 Ca2+ channels plays essential roles for various physiological events including thalamic oscillation, muscle contraction, hormone secretion, and sperm acrosomal reaction. In this study, we examined how protein tyrosine phosphatases or protein tyrosine kinases affect Cav3.2 Ca2+ channels reconstituted in Xenopus oocytes. We found that Cav3.2 channel activity was reduced by 25% in response to phenylarsine oxide (tyrosine phosphatase inhibitor), whereas it was augmented by 19% in response to Tyr A47 or herbimycin A (tyrosine kinase inhibitors). However, other biophysical properties of Cav3.2 currents were not significantly changed by the drugs. These results imply that Cav3.2 channel activity is capable of being increased by activation of tyrosine phosphatases, but is decreased by activation of tyrosine kinases.
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KEYWORD
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Cav3.2, Xenopus oocyte, protein tyrosine kinase inhibitor, protein tyrosine phosphatase inhibitor, voltage clamping
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